Nucleic acid amplification
Nucleic acid amplification significant chlamydial infection. Many commercial products that use nucleic acid amplification are now available. The test for nucleic acid intensify the nucleic acid target.
Image of nucleic acid amplification method.
No. 1.
• Advantages of Nucleic Acid Amplification technologies (NAAT).
• NAAT technology is more sensitive.
• NAAT nucleic test improve detention of infected purpose.
• Most effective method.
• Disadvantages of NAAT.
• High sensitivity of NAAT is a problem.
• NAAT test is more expensive.
No. 2.
• Staphylococus aureus. Developing a molecular diagnosis for HSC encapsulation.
• A lot of viruses causes the problem of “Central Nervous System” (CNS).
• Viral infection of diagnosis of CNS has revolutionized.
• The inflammatory reply engender by virus also classify consequences of both CSF pleocytosis and peripheral leukocytosis.
No. 2.
• Advantages PCR.
• PCR is suited in identifying living organism.
• The method can be carried out quick and it is ineffective.
• The method can be used in identifying the of CNS disease.
• Disadvantages.
• PCR technique is less widely available.
• It has unproven sensitivity.
No. 3.
• Suitable “Polymer Chain Reaction” PCR diagnosis.
• PCR diagnosis can be used to diagnosis any disease of those of nucleic acid ( example DNA and RNA).
• The PCR mostly used in detecting RNA and DNA virus in CFS.
Schematic diagram of the PCR process.
No. 3.
• Benefit of PCR technique
• This method can be used rapidly and less costly.
• CSF PCR bring positive result at a time of acute infection.
• Diagnosis less invasive than biopsy.
No. 4.
• Methicillis Resistant Staphylocus Aureus (MRSA).
• MRSA brought to people by methicillis resistant strain of MRSA.
• Some used MRSA strain and this seen disseminate very fast.
No. 4.
• Advantages of MRSA.
• “Pulsed- Field Gel Electrophoresis” (PFGE) are gold standard for typing molecular in MRSA.
• Disadvantages.
• Expensive and time consuming method.
• The method discriminatory to power and not applicable when there is power outbreak.
Pulsed Field Gel Electrophiresis
No. 5.
• Recombinant technology.
• Countries now a days attack each other by use of deadly technologies of deadly threat.
• They use virus and bacteria to attack each other.
• Recombinant technologies classified into 4 main phase which were used during world war.
No. 6.
• Cloning strategies for recombinant.
• Recombinant gene use fusion tags for purification.
• The simple and original cloning ways proposed for the purpose of getting amino acid sequence to original protein.
No. 9.
• Bio fuel product.
• Constant use of fossil fuel resulted in greenhouse gas emission.
• White biotechnology emphasis on the point of bio fuel.
No. 7.
• Strategies for purification of recombinant protein.
• Currently various range of of cloning vector obtained for the purpose of over expression in protein recombinant.
• Medium, pharmacy, biotechnology and food industries use technologies of Cloning Express System.
• 2 different purification strategies.
• Immobile Metal Affinity and Hydrophobic interaction Chromatographic.
No. 8.
• Next Gen sequence.
• Now days next gen has become on market for sometimes.
• The use of only one technologies does not produce the best result.•
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